addgene repository (Addgene inc)
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Addgene inc
addgene repository
Addgene Repository, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/addgene+plasmid+repository/pcDNA3%2E1-HA+(Plasmid+%23128034)/pm41840058-204-37-37
Average 95 stars, based on 82 article reviews
Addgene Repository, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/addgene+plasmid+repository/pcDNA3%2E1-HA+(Plasmid+%23128034)/pm41840058-204-37-37
Average 95 stars, based on 82 article reviews
addgene repository - by Bioz Stars,
2026-09
95/100 stars
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Plasmid Preparation:Article Title: Production of Genetically Engineered Extracellular Vesicles for Targeted Protein Delivery Article Snippet: .. NanoLuc-Jun_ pCMV plasmid is available through the Article Title: TGFβ-Smad3 signaling restores cell-autonomous Srsf1-mediated splicing of fibronectin in aged skeletal muscle stem cells Article Snippet: Myoblasts were exposed for 48 h to a concentration of 1-30 nM SIS3 (Sigma-Aldrich, 566405), or 20 ng/ml TGFβ1 (Biolegend, 763102) or EGF (R&D Technologies, 2028-EG) in the culture media. .. The FN minigene containing the EDB exon and flanking sequences was made available by Dr. Richard Hynes through the Article Title: Production of Genetically Engineered Extracellular Vesicles for Targeted Protein Delivery Article Snippet: .. EPN-Fos_pCMV plasmid is available through the Article Title: Single-step purification of functional Cas9 protein via the ubiquitin expression system. Article Snippet: The CRISPR/Cas9 system serves as a powerful platform for precise genome editing, with CRISPR/Cas9 ribonucleoprotein (RNP) complexes exhibiting superior editing efficiency compared to alternative delivery modalities.. However, current methods for Cas9 production typically involve multiple purification steps.. Here, we developed a streamlined single-step purification strategy for preparation of functional Streptococcus pyogenes Cas9 (SpCas9) with dispensable tag removal after inducible expression in Escherichia coli. Article Title: Design of highly functional genome editors by modelling CRISPR-Cas sequences. Article Snippet: .. The OpenCRISPR-1 enzyme and corresponding guide plasmids have been deposited to the Article Title: TGFβ-Smad3 signaling restores cell-autonomous Srsf1-mediated splicing of fibronectin in aged skeletal muscle stem cells. Article Snippet: Myoblasts were exposed for 48 h to a concentration of 1-30 nM SIS3 (Sigma-Aldrich, 566405), or 20 ng/ml TGFβ1 (Biolegend, 763102) or EGF (R&D Technologies, 2028-EG) in the culture media. .. Plasmid transfection and FN minigene assay The FN minigene containing the EDB exon and flanking sequences was made available by Dr. Richard Hynes through the Article Title: Design of highly functional genome editors by modelling CRISPR–Cas sequences Article Snippet: .. The OpenCRISPR-1 enzyme and corresponding guide plasmids have been deposited to the Article Title: Nanoscale engagement of programmed death ligand 1 (PD-L1) in membrane lipid raft domains of cancer cells. Article Snippet: 1 Department of Mathematical, Physical and Computer Sciences, University of Parma, Parma, Italy 2 Nanoscopy and NIC@IIT, Istituto Italiano di Tecnologia, Genoa, Italy 3 Department of Surgical, Medical and Molecular Pathology, and Critical Care Medicine, University of Pisa, Pisa, Italy 4 Unit of Clinical Pharmacology and Pharmacogenetics, Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy 5 Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy 6 Department of Translational Research & New Technologies in Surgery and Medicine, University of Pisa and Azienda Ospedaliero Universitaria Pisana, Pisa, Italy 7 Genoa Instruments s.r.l., Genoa, Italy 8 DIFILAB, Dipartimento di Fisica, Università degli Studi di Genova, Genoa, Italy 9 NEST Istituto Nanoscienze-CNR and Scuola Normale Superiore, Pisa, Italy 10 Dipartimento di Oncologia ed Emato-Oncologia, Università degli Studi di Milano “La Statale”, Milan, Italy Expressing:Article Title: Single-step purification of functional Cas9 protein via the ubiquitin expression system. Article Snippet: The CRISPR/Cas9 system serves as a powerful platform for precise genome editing, with CRISPR/Cas9 ribonucleoprotein (RNP) complexes exhibiting superior editing efficiency compared to alternative delivery modalities.. However, current methods for Cas9 production typically involve multiple purification steps.. Here, we developed a streamlined single-step purification strategy for preparation of functional Streptococcus pyogenes Cas9 (SpCas9) with dispensable tag removal after inducible expression in Escherichia coli. Clone Assay:Article Title: Single-step purification of functional Cas9 protein via the ubiquitin expression system. Article Snippet: The CRISPR/Cas9 system serves as a powerful platform for precise genome editing, with CRISPR/Cas9 ribonucleoprotein (RNP) complexes exhibiting superior editing efficiency compared to alternative delivery modalities.. However, current methods for Cas9 production typically involve multiple purification steps.. Here, we developed a streamlined single-step purification strategy for preparation of functional Streptococcus pyogenes Cas9 (SpCas9) with dispensable tag removal after inducible expression in Escherichia coli. Construct:Article Title: Single-step purification of functional Cas9 protein via the ubiquitin expression system. Article Snippet: The CRISPR/Cas9 system serves as a powerful platform for precise genome editing, with CRISPR/Cas9 ribonucleoprotein (RNP) complexes exhibiting superior editing efficiency compared to alternative delivery modalities.. However, current methods for Cas9 production typically involve multiple purification steps.. Here, we developed a streamlined single-step purification strategy for preparation of functional Streptococcus pyogenes Cas9 (SpCas9) with dispensable tag removal after inducible expression in Escherichia coli. Transfection:Article Title: TGFβ-Smad3 signaling restores cell-autonomous Srsf1-mediated splicing of fibronectin in aged skeletal muscle stem cells. Article Snippet: Myoblasts were exposed for 48 h to a concentration of 1-30 nM SIS3 (Sigma-Aldrich, 566405), or 20 ng/ml TGFβ1 (Biolegend, 763102) or EGF (R&D Technologies, 2028-EG) in the culture media. .. Plasmid transfection and FN minigene assay The FN minigene containing the EDB exon and flanking sequences was made available by Dr. Richard Hynes through the Mini Gene Assay:Article Title: TGFβ-Smad3 signaling restores cell-autonomous Srsf1-mediated splicing of fibronectin in aged skeletal muscle stem cells. Article Snippet: Myoblasts were exposed for 48 h to a concentration of 1-30 nM SIS3 (Sigma-Aldrich, 566405), or 20 ng/ml TGFβ1 (Biolegend, 763102) or EGF (R&D Technologies, 2028-EG) in the culture media. .. Plasmid transfection and FN minigene assay The FN minigene containing the EDB exon and flanking sequences was made available by Dr. Richard Hynes through the |